The use of serum separation gel to promote coagulation blood collection tube

How to correctly use separation gel to promote coagulation blood collection tubes to prepare high-quality serum samples, complete blood coagulation and centrifugation conditions are two crucial links, and centrifugation requires the use of a horizontal centrifuge.
The specific operation steps are as follows:
Immediately after blood collection, gently invert the blood collection tube 4 to 5 times to mix the specimens, wait for the specimens to fully coagulate and need to be placed for 30 minutes, the centrifugation radius is 200px, and the centrifugation speed is maintained at 3500-4000r/min for 10 minutes. Serum and blood clots are completely separated by separating gel, and serum samples can be directly tested on the machine or transferred to the test cups of the instrument. With this condition, high-quality serum samples can be prepared, indicating that the separation gel has a good effect. If the centrifugation speed is too low, the force acting on the separation gel is relatively weak, the separation gel does not turn well, or the blood is not completely coagulated and centrifuged, the fibrin coagulation may stay in the serum or the colloid layer, which may cause hemolysis. In addition to the emergency, the general biochemical examination is better after the blood is completely coagulated and centrifuged.