How to choose consumables for PCR laboratory (2)

The spread of the new crown epidemic in some areas has become a matter of most concern to everyone. The detection of the new coronavirus currently mainly uses the fluorescent quantitative PCR method, and the completion time of a nucleic acid test report takes about 4 to 5 hours. Viral nucleic acid can be used for detection only after extraction. Before performing instrument testing, it is necessary to go through the system construction work before the PCR experiment is put on the machine, and then transfer to the PCR machine to start the PCR experiment.

In the whole process, adding liquid to PCR seems to be a simple task, but at the current stage when the amount of detection is increasing sharply, it is actually a more labor-intensive link. Pipetting users usually have many considerations when choosing a measuring measuring pipette, but when choosing a measuring measuring pipette tip, they don’t pay enough attention to it. The measuring measuring pipette tip is like the bullet of a measuring measuring pipette gun, which has a great impact on the accuracy of pipetting, so the importance is not lost. measuring measuring pipette gun. Little sucker, ask the university, let’s learn together.

1 What information do we need to know to buy a tip?

“As long as the tip that can be installed is the tip that can be used”-this is almost all users’ common understanding of the adaptability of the tip. This statement can be said to be partly true but not entirely true.

The tip that can be mounted on the measuring measuring pipette can indeed form a pipetting system with the measuring measuring pipette to realize the pipetting function, but is this reliable? A question mark is required here. Answering this question requires data to speak.

(1) You may wish to perform a performance test after matching the measuring measuring pipette with the tip. After rinsing the tip, perform several repeated sample addition operations, weigh the sample addition amount each time, and record the reading.

(2) Calculate the accuracy and precision of the pipetting operation after converting it into volume according to the density of the test liquid.

(3) What we have to choose is a tip with good and more precise accuracy. If the measuring measuring pipette and the measuring measuring pipette are matched with poor accuracy, it means that the tightness of the measuring measuring pipette and the measuring measuring pipette cannot be guaranteed, so that the results of each operation cannot be reproduced.

2 What information do we need to know to buy a tip?

The performance indicators of the tip are: low adsorption, filter element, tightness, force of loading and ejection, no DNase and RNase, no pyrogen; These indicators can be obtained from the following information.

01 Material quality At present, the suction tips on the market are basically made of polypropylene plastic (colorless and transparent plastic with high chemical inertness and wide temperature range). High-quality tips are generally made of natural polypropylene, while cheap tips are likely to use recycled polypropylene plastic. The tip provided by Huida is made of imported polypropylene material that meets USP Class-VI standards. It has high transparency, resistance to organic solvents, and a wide range of temperature resistance: -80℃~121℃, no deformation after high temperature and high pressure, high-quality raw materials Bring high performance!

In addition, most of the tips will add a small amount of additives during the manufacturing process. Common ones are:

(1) color-developing materials, used for blue and yellow tips;

(2) additives. Help the tip to be separated from the mold quickly after forming.

(3) Additives for treating the surface of the low-adsorption gun tip.

02  Biological pollution

More and more users will require that the tips are RNase-free, DNase-free, DNA-free, pyrogen-free and ATP-free. However, high-temperature sterilized tips cannot completely guarantee the absence of biological contamination. To ensure that there is no biological contamination, steps such as gamma ray treatment are required. Therefore, it is almost impossible for users to dispose of ordinary tips into non-biologically contaminated tips, and the purchased non-biologically contaminated boxed tips must provide corresponding certificates to indicate the test data of the corresponding items. The tips provided by Doheng are all produced in a 100,000-class dust-free workshop to ensure that the products are pyrogen-free, DNase-free, and RNase-free.

03 Tips category and specifications

In addition to the most commonly used standard tips, there are also filter tips, low-adsorption tips, automatic workstation tips, and wide-mouth tips, which are suitable for different experiments. For example, the sample measuring measuring pipetted by the filter tip cannot enter the inside of the measuring measuring pipette, thereby protecting the parts of the measuring measuring pipette from contamination and corrosion. More importantly, it can also ensure that there will be no cross-contamination between samples.

The packaging specifications of the tip are mainly in bags and boxes. The box is sterilized before leaving the factory, and the user can use it directly; there are 500 or 1000 bagged tips per bag, and most users will buy the bagged tips and then put the tips into the tip box . Boxed packaging is relatively expensive, but bag packaging also increases the risk of contamination during the process of manually loading the tip box.

Huida has a complete range of tips, both in bags and boxes. Pipetting specifications: 10ul, 100ul, 200ul, 300ul, 1000ul, 1250ul; there are also ordinary tips, filter tips, and conductive tips suitable for workstations. . As a professional supplier of medical equipment & consumables, Huida can not only provide customers with overall solutions for PCR instruments and equipment, and strive for PCR laboratory consumables, we can provide a full range of high-quality products and related services. Welcome to send an inquiry for more details.