How to choose consumables for PCR laboratory(1)

Since the new crown epidemic, nucleic acid testing has received more and more attention, and the demand for PCR laboratories has also increased. Today, I will mainly introduce how to choose consumables for PCR laboratory.

First of all, we need to clarify what kind of PCR experiment we are doing. According to the different detection methods, PCR experiments are divided into ordinary PCR method and fluorescence quantitative PCR method. The former is generally determined by the end-point PCR running gel method. Therefore, when choosing consumables, the main thing to pay attention to is the thermal conductivity and sealing of the consumables themselves; while the latter It is the real-time detection of fluorescent signals. In addition to thermal conductivity and airtightness, there are also requirements for light transmittance and light resistance. In other words, the consumables that can be used for real-time quantitative PCR must be used for ordinary PCR. On the contrary, the effect will be much worse!

Tightness

The better the sealing, the less the product volatilizes, the more products are obtained, and the less aerosols are produced, and the less pollution for the next round of experiments, which means the lower the false positive rate. .

Thermal conductivity

The better the thermal conductivity and the more uniformity, the faster the PCR speed and the higher the accuracy.

Light transmittance

This is mainly for fluorescence quantification. We know that it is a real-time quantitative method. The light transmittance of the consumable itself is directly related to the amount of fluorescent signal we detect. If the selected consumable has insufficient light transmittance, it should be If the detected signal is not detected, it will cause us to misjudge the result.

The full line of PCR series laboratory consumables provided by Jiangsu Huida Medical Equipment in China are injection molded and assembled in a 100,000-level purification workshop. In addition to ensuring that there is no DNase, RNase, and heat source, they are also in airtightness, thermal conductivity, and light transmittance. , Light resistance and other aspects are relatively good on the market.

PCR plate

Different from the bottom injection molding method of other manufacturers on the market, the injection point of this PCR plate is at the intersection of the hole and the hole on the plate surface, so that the bottom of the PCR plate has better contact with the PCR machine, and the purity is as high as 99.9 % Polypropylene, ultra-thin design, uniform wall thickness, accurate heat conduction, accurate temperature control, and effectively reduce the experimental cycle time.

PCR tube

Adopting unique one-shot injection molding technology, ultra-thin design, fast heat conduction, window lower than flat surface, not easy to be scratched, better sealing of the tube, almost zero evaporation, reducing aerosol pollution, and avoiding false positives to the greatest extent . Among them, the fluorescent series, the lid is highly transparent, and can be applied to two detection methods of top reading and bottom reading.

PCR 8 Strips tube

The design of the high-perspective window below the plane can avoid scratches to the greatest extent. The use of 100% imported raw materials allows the tube wall to have uniform flatness and stable non-sticking properties, ensuring the accuracy of sample processing and suction.

Jiangsu Huida Medical Equipment Co., Ltd.

Jiangsu Huida Medical Equipment Co., Ltd., as a professional supplier of medical equipment & consumables, can not only provide customers with overall solutions for PCR instruments and equipment, and strive for PCR laboratory consumables, we can provide a full range of high-quality products and related services. Welcome to send an inquiry to learn more.