Are there any specific ways of dealing with slides that have colored backgrounds or transparent materials and how do they affect experimental results?

Special treatments for slides with colored backgrounds or transparent materials and their effects on experimental results are mainly based on scattering, absorption and color perception. These factors synergistically work on the experimental result leading to color deviations and variation in visual effects.

Scattering and absorption phenomena:

Scattering and absorption of light within material can cause changes in propagation path as well as intensity. For example, light is scattered internally numerous times when it passes through a semi-transparent medium which means that light distribution changes too, hence causing its color presentation to be changed. Moreover, the color of a beam also varies due to both absorbance and scattering when it goes through materials possessing different optical properties.

Changes in color perception:

Besides physical features, color perception is influenced by psychological state of an observer as well as environmental conditions. When a picture with differently chromaticity substrate is printed out, person’s colour perception may be affected since its colour has been partly adapted to his/her visual system. Additionally, object colors mixed with background colors also alter the perceived transparency for instance; mixing ratio between background color and object color determines perceptual transparency of clear layers.

Impact of experimental results:

The above-mentioned outcomes could lead to errors in experiments’ precision and reliability because they induce variations in colours that result from these deviations amongst others. However if materials or background colors used during these tests are significantly different from what was expected then the experiment will not be accurate while carrying out colour matching/ colour reproduction experiments. Again readings from measuring instruments can also be affected by the optical properties i.e., whiteness measurement of fluorescent substances is dependent upon UV content present in light source shimmering down upon them.

Such special manipulations should be considered about these slides made up of colored background or transparent material during various experiments done on them.

References

1. J. Sjollema, Ir. T. den Exter et al. “Measurement of color and scattering phenomena of translucent materials.” Other Conferences (1991).

2. A. Walter, M. Schuerer et al. “Objective approach for measuring changes in color discrimination caused by transparent colored filter media..” Journal of The Optical Society of America A-optics Image Science and Vision (2009). 2219-25.

3. Alejandro Ferrero, E. Perales et al. “Color representation and interpretation of special effect coatings..” Journal of The Optical Society of America A-optics Image Science and Vision (2014). 436-47.

4. Y. Ito and K. Uchikawa. “The Effects of Chromatic Conditions for Transparency.” (1997). 193 – 193.

5. P. Green and Boris Oicherman. “Reproduction of colored images on substrates with varying chromaticity.” IS&T/SPIE Electronic Imaging (2003).

6. Juan Lin, R. Shamey et al. “Factors affecting the whiteness of optically brightened material..” Journal of The Optical Society of America A-optics Image Science and Vision (2012). 2289-99 .

7. B. Kruse and S. Gustavson. “Rendering of color on scattering media.” Electronic imaging (1996).